A Molecular Ruler for Measuring Quantitative Distance Distributions
نویسندگان
چکیده
منابع مشابه
A Molecular Ruler for Measuring Quantitative Distance Distributions
We report a novel molecular ruler for measurement of distances and distance distributions with accurate external calibration. Using solution X-ray scattering we determine the scattering interference between two gold nanocrystal probes attached site-specifically to a macromolecule of interest. Fourier transformation of the interference pattern provides a model-independent probability distributio...
متن کاملMeasuring the distance between two mercapto groups with an optical molecular ruler on the nanometer scale.
We have developed an absorption spectrum based molecular ruler that measures the distance between two mercapto groups. The conformation of the molecular rulers changes with the distance, which induces an absorption spectrum change. DFT calculation has been carried out to elucidate the relation between the molecular conformation and the absorption spectrum. With this simple method, we can estima...
متن کاملA nanoplasmonic molecular ruler for measuring nuclease activity and DNA footprinting.
GANG L. LIU, YADONG YIN2,3, SIRI KUNCHAKARRA, BIPASHA MUKHERJEE, DANIELE GERION, STEPHEN D. JETT, DAVID G. BEAR, JOE W. GRAY, A. PAUL ALIVISATOS, LUKE P. LEE* AND FANQING FRANK CHEN* Biomolecular Nanotechnology Center, Department of Bioengineering, University of California at Berkeley, Berkeley, California 94720, USA Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA Departm...
متن کاملA PELDOR-based nanometer distance ruler for oligonucleotides.
A pulsed electron paramagnetic resonance (EPR) spectroscopic ruler for oligonucleotides was developed using a series of duplex DNAs. The spin-labeling is accomplished during solid-phase synthesis of the oligonucleotides utilizing a palladium-catalyzed cross-coupling reaction between 5-iodo-2'-deoxyuridine and the rigid spin-label 2,2,5,5-tetramethyl-pyrrolin-1-yloxyl-3-acetylene (TPA). 4-Pulse ...
متن کاملMeasuring ubiquitin chain linkage: Rap80 uses a molecular ruler mechanism for ubiquitin linkage specificity
DNA double-stranded breaks lead to the recruitment of the Rnf8–Ubc13 complex to the damage site where they catalyse K63-linked ubiquitination. Receptor-associated protein 80 (Rap80) subsequently binds the K63-linked chains and brings with it a BRCA1 complex. Sato et al (2009) solve the crystal structure of the Rap80-UIM1UIM2:K63-linked diubiquitin complex to show that a contiguous helix spans t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2008
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0003229